Application

Research CategoryMetabolism

Specifically binds to and precipitates Ras-GTP from cell lysate.Can be bound to glutathione coated plates prior to precipitating Ras-GTP.

Research Sub CategoryObesityMetabolic Disorders

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity >50% at full length molecular weight 42 kDa.
Specifically binds to and precipitates Ras-GTP from cell lysate. Can be bound to glutathione coated plates prior to precipitating Ras-GTP.

Product Source: Expressed in E. coli

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Glutathione-agarose

300 µg of Raf-1 RBD, GST in 150 µL in PBS, pH 7.4, 0.5mM PMSF, and 20% glycerol.

Quality

Affinity Precipitation of Activated Ras: Raf-1-RBD, GST can be captured on a glutathione coated plate and used to capture GTP activated Ras from HeLa cell lysate. Used 2 µg of Raf-1-RBD, GST per well to capture 25-100 µg of EGF stimulated HeLa cell lysate. Upstate also offers a Ras GTPase ELISA kit (17-424).
Note: Due to spontaneous deactivation of Ras, use lysates that have been stored at -70°C for less than 6 month.

Storage and Stability

1 year at -20°C from date of shipment.